Ved: 6 June 2012 / Accepted: 18 November 2012 / Published on the net: 15 December 2012 ?Springer Science+Business Media DordrechtAbstract Gliomas can diffuse in to the regular brain and this invasion of glioma cells involves modification of receptor-mediated adhesive properties of tumor cells, degradation and remodeling of extracellular matrix by tumor-secreted metalloproteinase (MMPs) including MMP-2, consequently generating an intercellular space for invasion of glioma cells. BmK CT, one of the key toxins in scorpion Buthus martensii Karsch venom, is often a novel blocker on the chloride ion channel and MMP-2. In this report, a recombinant plasmid pEGFP-N1-BmK CT was constructed and characterized by in vitro research. The results showed that pEGFP-N1 mediated BmK CT expression displayed a higher activity in suppressing cell migration by means of MMP-2. The prospective therapeutic impact of pEGFP-N1 mediated BmK CT against rat glioma C6 cells was assessed and its prospective mechanism was elucidated. It represented an strategy for building a novel therapeutic agent–recombinant plasmid pEGFP-N1-BmK CT as an efficient and highly effective adjuvant. Keywords and phrases BmK CT ?Glioma cells ?Matrix metalloproteinase-2 ?MigrationIntroduction Glioma is really a hugely invasive, swiftly spreading form of brain cancer that’s resistant to surgical and healthcare remedy. Malignant gliomas will be the most important brain tumors notoriously resistant to at the moment accessible therapies, because they fail to undergo apoptosis upon anticancer therapy (Wang and Ji 2005). Classic therapy of brain tumors involves surgery, radiation therapy, chemotherapy, and immunotherapy (Yin et al. 2007). Despite these treatment options, the median survival time is significantly less than 15 months (Demuth and Berens 2004). It is clear that some adjuvant therapy will bring about newer and more effective techniques and techniques for brain tumor handle in the future. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endoproteinases involved inside the degradation from the extracellular matrix (ECM). The expression amount of MMPs correlates with all the invasion of cancer cells because the degradation of the ECM is crucial for tumor angiogenesis, invasion and metastasis (Gladson 1999; Shao et al. 2011; Li et al. 2011; Lee et al. 2011). Of note, MMP-2 is up-regulated in gliomas and related cancers, but its expression level is trivial in typical brain tissue (Deshane et al. 2003), which demonstrates that MMP-2 could be regarded as a possible crucial target for glioma suppression. Chlorotoxin (Cltx), one of the key toxins in scorpion Leiurus quinquestriatus venom, has been shown to bind particularly to glioma cell surface as a particular chloride channel blocker (Shen et al.470482-44-1 Order 2005;Y.Price of 6-Chloro-7-deazapurine-β-D-riboside Fu ( ) ?Y.PMID:25429455 Jiao ?N. An ?A. Liang Crucial Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, People’s Republic of China e-mail: [email protected] (2013) 65:533?Yang et al. 2005; Burton et al. 2008). Benefits from matrigel invasion assay demonstrate that Cltx can inhibit the enzymatic activity of MMP-2 that is involved in cell migration and particularly up-regulated in gliomas (Deshane et al. 2003). A chlorotoxinlike peptide gene, BmK CT, was cloned and sequenced from the venom of B. martensii Karsch by Wu et al. (2000) and Zeng et al. (2000). Similarly, the significance of BmK CT has been properly documented as a novel blocker of chloride channel and MMP-2 (Fu et al. 2007). Compared with o.