And data collection. Crystal kinds 1 and two ?diffracted to 1.85 A resolution at SPring-8 (Figs. 3a and 3b). Crystal ?kind 3 diffracted to 2.5 A resolution on in-house X-ray evaluation (Fig. 3c). Data-collection and processing statistics are shown in Table 1. The crystals belonged to space groups P6322, P21 and P1, ?with unit-cell parameters a = b = 143.60, c = 84.54 A, a = 114.54, ?, = 94.99 and a = 94.45, b = 94.96, b = 105.82, c = 116.67 A ?c = 100.66 A, = 107.02, = 108.44,= 110.71 , respectively. The calculated Matthews coefficients (VM) had been 2.41, two.25 and ?two.28 A3 Da?, with corresponding solvent contents of 0.49, 0.45 and 0.48, assuming the presence of 1, six and six subunits inside the asymmetric units, respectively (Matthews, 1968). In 129, 250 and 1392 frames, totals of 599 739, 1 443 947 and 125 424 reflections corresponding to 43 968, 235 443 and 87 040 exceptional reflections had been collected with 99.4, 99.9 and 84.6 completeness and Rmerge or RsymFigureX-ray diffraction images of soybean A1bB2 glycinin crystals. (a) Diffraction image of crystal sort 1, (b) diffraction image of crystal form two and (c) diffraction image of crystal ?type three. The outer black circles in (a) and (b) correspond to 1.85 A resolution.Prak et al.Soybean mature glycinin A1bBActa Cryst. (2013). F69, 937?crystallization communications?values of 0.062, 0.059 and 0.083 to 1.85, 1.85 and 2.50 A resolution for crystal kinds 1, two and 3, respectively. Study is in progress to figure out the three-dimensional structure of A1bB2. This operate was supported in part by a grant from the National Project on Protein Structural and Functional Analyses from the Ministry of Education, Culture, Sport, Science and Technology of Japan.Price of 2096419-56-4 Diffraction information for crystals 1 and two have been collected in the BL41XU and BL38B1 stations of SPring-8 (Hyogo, Japan) with the approval of JASRI (proposal Nos. 2007A1489, 2007B1490, 2008A1263 and 2008B1574). We would prefer to thank Dr Jamie Freeman (LMCB, Healthcare Investigation Council, UCL) for his vital reading of this manuscript.Holzhauser, T., Wackermann, O., Ballmer-Weber, B. K., Bindslev-Jensen, C., Scibilia, J., Perono-Garoffo, L., Utsumi, S., Poulsen, L. K. Vieths, S. (2009). J. Allergy Clin. Immunol. 123, 452?58. ????Ito, K., Sjolander, S., Sato, S., Moverare, R., Tanaka, A., Soderstrom, L., Borres, M., Poorafshar, M. Ebisawa, M. (2011). J. Allergy Clin. Immunol. 128, 673?75. Kito, M., Moriyama, T., Kimura, Y. Kambara, H. (1993). Biosci. Biotechnol.Aminoethyl-SS-propionic acid supplier Biochem.PMID:24631563 57, 354?55. Maruyama, N., Prak, K., Motoyama, S., Choi, S.-K., Yagasaki, K., Ishimoto, M. Utsumi, S. (2004). J. Agric. Meals Chem. 52, 8197?201. Matthews, B. W. (1968). J. Mol. Biol. 33, 491?97. Otwinowski, Z. Minor, W. (1997). Procedures Enzymol. 276, 307?26. Prak, K., Maruyama, Y., Maruyama, N. Utsumi, S. (2006). Peptides, 27, 1179?186. Prak, K., Nakatani, K., Katsube-Tanaka, T., Adachi, M., Maruyama, N. Utsumi, S. (2005). J. Agric. Meals Chem. 53, 3650?657. Prak, K., Nakatani, K., Maruyama, N. Utsumi, S. (2007). Protein Eng. Des. Sel. 20, 433?42. Prak, K. Utsumi, S. (2009). J. Agric. Food Chem. 57, 3792?799. Shirzadegan, M., Christie, P. Seemann, J. R. (1991). Nucleic Acids Res. 19, 6055. Staswick, P. E., Hermodson, M. A. Nielsen, N. C. (1984). J. Biol. Chem. 259, 13424?3430. Tandang, M. R., Atsuta, N., Maruyama, N., Adachi, M. Utsumi, S. (2005). J. Agric. Food Chem. 53, 8736?744. Utsumi, S. (1992). Adv. Meals Nutr. Res. 36, 89?08. Utsumi, S., Matsumura, Y. Mori, T. (1997).